Use this oligo dilution calculator tool to find the dilution of oligo solutions. Just enter the stock solution concentration and required working concentration along with required volume and you will get how much stock solution you need to dilute in diluent (water or buffer) to create a working solution.

The Oligo Dilution Calculator is a useful tool designed to help scientists and researchers dilute an initial stock solution of an oligonucleotide (oligo) to a desired final concentration and volume. This is particularly important in molecular biology experiments where precise concentrations of oligos are required for PCR, sequencing, or other applications.

Principles

Dilution is the process of reducing the concentration of a solute in a solution, usually by adding more solvent. The principle behind this calculator is based on the dilution equation:

C1V1=C2V2

Where:

• C1 is the initial concentration (stock concentration) of the oligo.
• V1 is the volume of the stock solution needed.
• C2 is the final concentration of the oligo.
• V2 is the final total volume of the diluted solution.

Uses

1. Preparing Working Solutions: When conducting experiments, it is often necessary to prepare working solutions of oligonucleotides from a more concentrated stock solution. This calculator helps in determining the exact amount of stock solution and diluent needed.
2. Ensuring Accuracy: Accurate dilution is critical in experiments to ensure reproducibility and validity of results. This calculator assists in minimizing errors associated with manual calculations.
3. Time-Saving: By automating the dilution calculation, researchers can save time and reduce the risk of calculation errors.

How to Use the Calculator

1. Initial Stock Concentration: Enter the concentration of your initial stock solution and select the appropriate unit (Molar, Millimolar, Micromolar, Nanomolar, Picomolar).
2. Final Desired Concentration: Enter the concentration you want for your final solution and select the appropriate unit.
3. Final Volume: Enter the final volume of the diluted solution you need and select the appropriate unit (Microliters, Milliliters, Liters).
4. Calculate: Click the ‘Calculate’ button to determine the volume of the stock solution needed and the volume of diluent required to achieve the desired final concentration and volume.

Equations Used

The primary equation used in this calculator is the dilution equation:

C1V1=C2V2

Rearranging this equation to solve for V1V1​ (the volume of the stock solution needed):

V1=C2×V2/C1

Once V1 is calculated, the volume of diluent required (Vdiluent​) can be determined by subtracting the volume of the stock solution from the final volume:

Vdiluent=V2−V1

Example Calculation

Suppose you have a stock solution of an oligonucleotide at 100 µM, and you need 50 µL of a 10 µM solution:

1. Initial Stock Concentration: 100 µM
2. Final Desired Concentration: 10 µM
3. Final Volume: 50 µL

Using the dilution equation:

V1=C2×V2/C1

V1=10×50/100

V1=5 µL

So, you need 5 µL of the 100 µM stock solution.

To find the volume of diluent needed:

Vdiluent=V2−V1

Vdiluent=50 µL−5 µL

Vdiluent=45 µL

You would mix 5 µL of your stock solution with 45 µL of diluent to obtain 50 µL of a 10 µM solution.

The Oligo Dilution Calculator is a straightforward and essential tool for accurately preparing diluted solutions of oligonucleotides. By understanding and utilizing the dilution principles and equations, researchers can ensure precise concentrations necessary for successful experiments.