Punnett Square Calculator

The Punnett Square Calculator is an intuitive genetic calculator for predicting genetic inheritance patterns. To use it, select the number of traits you want to calculate i.e. monohybrid cross, dihybrid cross or try-hybrid cross. This calculator can show you calculations up to 5 traits. Then enter the genotypes of both parents for the number of traits selected.

The calculator will generate a Punnett Square, showing all possible allele combinations and their probabilities. Punnett square solver will fill in the grid with potential genotypes in color coded pattern for the offspring and provide a summary of genotypic and phenotypic ratios. This tool is ideal for understanding the inheritance of traits like cystic fibrosis.

Punnett Square Calculator

Punnett Square Calculator

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Understanding the Punnett Square Calculator

The Punnett Square Calculator is a valuable tool for anyone interested in the principles of genetic inheritance. It simplifies the complex process of predicting how traits will be passed from parents to offspring.

This tool is not only useful for predicting simple traits like blood groups but also for understanding genetic disorders such as cystic fibrosis. In this guide, we’ll explain how the Punnett Square Calculator works, its various applications, and the fundamental genetic concepts you need to know.

How to Create a Punnett Square

Creating a Punnett square is a straightforward process that helps visualize the combination of genes from parents to offspring. Here’s a detailed step-by-step guide to help you get started:

Step 1: Determine the Parent Genotypes

First, choose the number of traits you want to work with. Then identify the genetic makeup (genotype) of both parents. This includes determining whether they are homozygous (possessing two identical alleles, such as BB or bb) or heterozygous (possessing two different alleles, such as Bb) for a particular trait.

Step 2: Set Up the Square

Draw a grid with four boxes. On the top, write down all possible allele combinations that one parent can contribute to its gametes (sperm or egg cells). Do the same for the other parent along the left side of the grid. For instance, if both parents are heterozygous (Bb) for a trait, the top row and the left column will each have “B” and “b”.

Step 3: Fill in the Squares

Combine the alleles from each row and column to fill in the squares of the grid. Each square represents a possible genotype for the offspring. For example:


In this example, there are four possible genotypes for the offspring: BB, Bb, Bb, and bb.

Example: Predicting Eye Color

Suppose eye color is determined by a single gene with two alleles: B (brown, dominant) and b (blue, recessive). If both parents are heterozygous (Bb), the Punnett square helps predict the potential genotypes and phenotypes of their children.

Genotypic Ratio:

  • 1 BB : 2 Bb : 1 bb

Phenotypic Ratio:

  • 3 Brown : 1 Blue

This means there is a 75% chance their child will have brown eyes and a 25% chance their child will have blue eyes.

Practical Application: Using the Calculator

Let’s consider a practical example involving cystic fibrosis (CF), a genetic disorder inherited in an autosomal recessive pattern. This means a child must inherit two copies of the defective gene (one from each parent) to develop the disease.


  • Both parents are carriers (heterozygous) of the CF gene (Ff).

The Punnett square would be set up as follows:


Genotypic Ratio:

  • 1 FF : 2 Ff : 1 ff

Phenotypic Outcome:

  • 75% healthy (FF or Ff), 25% affected (ff)

This indicates that there is a 75% chance that their child will be healthy (either not carrying the CF gene or being a carrier) and a 25% chance that their child will have cystic fibrosis.

Understanding Genotypic and Phenotypic Ratios


  • The genetic makeup of an organism, such as FF, Ff, or ff.


  • The observable traits resulting from the genotype, such as having brown eyes or blue eyes, or being healthy versus having cystic fibrosis.

In the example above, the phenotypic ratio shows that despite both parents being carriers they have a 25% chance of having kid with cystic fibrosis, and the majority of offspring will be healthy, although some may be carriers of the recessive gene.

By following the steps outlined above, you can accurately predict the likelihood of various genetic outcomes and gain deeper insights into the fascinating world of genetics.

Key Genetic Concepts

Homozygous: When an organism has two identical alleles for a specific trait, it is termed homozygous. This can be represented by two dominant alleles (e.g., AA) or two recessive alleles (e.g., aa). Homozygous organisms will consistently pass on the same allele for a trait to their offspring.

Heterozygous: An organism is heterozygous for a trait when it has two different alleles for that trait (e.g., Aa). Heterozygous organisms have one dominant and one recessive allele, and they can pass either allele to their offspring.

Dominant Allele: A dominant allele is one that expresses its trait even when paired with a different allele. It is typically represented by a capital letter (e.g., A). In a heterozygous pairing (Aa), the trait associated with the dominant allele will be visible.

Recessive Allele: A recessive allele is one whose trait is masked by the presence of a dominant allele. It is represented by a lowercase letter (e.g., a). The trait associated with a recessive allele only appears when an organism has two copies of the recessive allele (aa).

Mendelian Inheritance

Gregor Mendel’s Contributions: Gregor Mendel, often referred to as the Father of Genetics, conducted experiments with pea plants in the mid-19th century. Through his work, he discovered fundamental principles of heredity, including the concepts of dominant and recessive alleles. Mendel’s experiments demonstrated how traits are passed from parents to offspring and laid the foundation for the laws of segregation and independent assortment.

Types of Punnett Squares

A single-trait Punnett square tracks two alleles for each parent and has two rows and two columns. Adding more traits increases the size of the Punnett square. Assuming all traits exhibit independent assortment, the number of allele combinations an individual can produce is two raised to the power of the number of traits. For two traits, an individual can produce four allele combinations (2^2). Three traits produce eight combinations (2^3).

Monohybrid Cross: A monohybrid cross involves a single trait. For example, if both parents are heterozygous for a trait (Bb x Bb), the Punnett square will have four boxes, showing the possible combinations of alleles their offspring might inherit.

Dihybrid Cross: A dihybrid cross involves two traits. For example, if both parents are heterozygous for two traits (BbCc x BbCc), the Punnett square becomes more complex, with sixteen boxes representing all possible allele combinations for the two traits.

Trihybrid Cross: A trihybrid cross involves three traits, further increasing the complexity of the Punnett square. With three traits, the Punnett square will have sixty-four boxes, representing all possible allele combinations for the three traits.

Example: Dihybrid Cross

In a dihybrid cross where both parents are heterozygous for two traits (e.g., AaBb x AaBb), the Punnett square can predict the phenotype ratios of the offspring. Assuming complete dominance for both traits, the phenotypic ratio expected is 9:3:3:1. This means out of sixteen possible combinations:

  • 9 combinations will have both dominant phenotypes.
  • 3 combinations will have one dominant and one recessive phenotype.
  • 3 combinations will have the other dominant and recessive phenotype.
  • 1 combination will have both recessive phenotypes.

This ratio illustrates Mendel’s principle of independent assortment, provided the traits are not linked on the same chromosome.

Understanding these genetic concepts and how to apply Punnett squares helps in predicting the inheritance patterns of traits. By exploring the complexities of genetic combinations and the principles of Mendelian inheritance, one can gain deeper insights into the mechanisms that drive heredity and variation within populations.

Independent Assortment and Linked Genes

Punnett squares typically assume that genes assort independently, meaning the inheritance of one gene does not affect the inheritance of another.

The Complexity of Linkage

However, in reality, genetic inheritance can be more complex due to linkage. Linked genes are located close together on the same chromosome and tend to be inherited together because they do not assort independently. This can significantly alter the expected ratios of offspring phenotypes.

The Role of Recombination

Recombination, or crossing over, can occur during meiosis, where homologous chromosomes exchange segments. This process can separate linked genes and create new combinations of alleles. The likelihood of recombination between two genes increases with the distance between them on the chromosome.

Integrating Linkage and Recombination into Punnett Squares

To accurately predict offspring genotypes when dealing with linked genes, Punnett squares must be adjusted to account for the probability of recombination.


Consider a dihybrid cross involving two linked genes, A and B, with parental genotypes AB/ab and AB/ab. If there is no recombination, the gametes will be either AB or ab, resulting in:


If recombination occurs with a frequency of 20%, we will have four types of gametes: AB (40%), ab (40%), Ab (10%), and aB (10%). The Punnett square then will need to account for that.

This adjusted Punnett square accounts for both linkage and recombination, providing a more accurate prediction of offspring genotypes.

Understanding these factors allows for more precise predictions and a deeper insight into the mechanisms of heredity.

Multiple Alleles and Co-dominance

While the Punnett square method typically deals with two alleles for each trait, some traits are controlled by more than two alleles. For example, human blood type is determined by three alleles: A, B, and O. These alleles can combine in different ways to produce four possible blood types (A, B, AB, and O).

In the case of co-dominance, where both alleles in a heterozygous organism are fully expressed, the Punnett square must be adapted to account for these variations. For example, in blood types:

  • A person with genotype AA or AO will have type A blood.
  • A person with genotype BB or BO will have type B blood.
  • A person with genotype AB will have type AB blood (co-dominance).
  • A person with genotype OO will have type O blood.

By incorporating these complexities, the Punnett Square Calculator can help you predict outcomes for a wider array of genetic scenarios.

FAQ: Common Questions About Punnett Squares

How do I use a Punnett square?

  1. Select a monohybrid, dihybrid, trihybrid, tetrahybrid or pentahybrid punnet cross.
  2. Determine the genotypes of both parents.
  3. Set up the square with one parent’s alleles on top and the other’s on the side.
  4. Fill in the squares to see potential offspring genotypes.

How can I tell if a genotype is heterozygous or homozygous?

  • Heterozygous: One dominant and one recessive allele (Aa).
  • Homozygous: Two identical alleles, either dominant (AA) or recessive (aa).

What is the purpose of a Punnett square?

Punnett squares help predict the probability of an offspring inheriting specific traits, providing insights into genetic combinations and aiding in understanding genetic disorders.

Limitations of Punnett Square Solver

Using the Punnett Square Calculator, you can explore various genetic scenarios, enhancing your understanding of inheritance patterns and the principles of genetics. Whether for educational purposes or practical genetic counseling, this tool is invaluable in simplifying complex genetic predictions. But, given the complexity of genetics, Punnett squares are not always the best method for calculating genotype and phenotype ratios for crosses involving multiple trait due to phenomena like linkage and recombination. For complex scenarios with multiple traits and alleles, advanced genetic tools and techniques may be more appropriate.

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