The Oligo Resuspension Calculator is a valuable tool designed to help scientists and researchers prepare a stock solution from a dry oligonucleotide (oligo).

This calculator simplifies the process of dissolving a dry oligo into a specific concentration and volume, ensuring accuracy and efficiency in molecular biology experiments.

Uses

1. Preparing Stock Solutions: When conducting experiments, it is often necessary to prepare stock solutions of oligonucleotides from dry oligos provided by suppliers. This calculator helps determine the exact volume of diluent required.
2. Ensuring Accuracy: Accurate resuspension is critical in experiments to ensure reproducibility and validity of results. This calculator assists in minimizing errors associated with manual calculations.
3. Time-Saving: By automating the resuspension calculation, researchers can save time and reduce the risk of calculation errors.

How to Use the Calculator

1. Delivered Amount of Dry Oligo: Enter the amount of dry oligo in the tube and select the appropriate unit (Micromoles, Nanomoles, Picomoles).
2. Desired Stock Concentration: Enter the concentration you want for your stock solution and select the appropriate unit (Micromolar, Nanomolar, Picomolar).
3. Calculate: Click the ‘Calculate’ button to determine the volume of diluent needed to achieve the desired stock concentration.

Principle

Resuspension involves dissolving a dry oligonucleotide in a suitable buffer or solvent to create a stock solution of a desired concentration. The principle behind this calculator is based on the equation:

V=m/C

Where:

• V is the volume of diluent required to resuspend the dry oligo.
• m is the amount of dry oligo.
• C is the desired stock concentration.

The volume of diluent needed (V) is calculated by dividing the amount of dry oligo (m) by the desired stock concentration (C).

Example Calculation

Suppose you have 5 nmol of dry oligo and you want to prepare a 100 µM stock solution:

1. Delivered Amount of Dry Oligo: 5 nmol
2. Desired Stock Concentration: 100 µM

Using the resuspension equation:

V=m/C 

V=5 nmol/100 µM

V=5×10⁻⁹ mol/100×10⁻⁶ mol/L

V=0.00005 L

V=50 µL

So, you need 50 µL of diluent to resuspend the 5 nmol of dry oligo to prepare a 100 µM stock solution.

The Oligo Resuspension Calculator is a straightforward and essential tool for accurately preparing stock solutions of oligonucleotides. By understanding and utilizing the resuspension principles and equations, researchers can ensure precise concentrations necessary for successful experiments.

Important Notes

• Oligos should be resuspended in TE Buffer (10 mM Tris-HCl / 1 mM EDTA), pH 8.0 (recommended) or DNase-free water.
• Ensure all measurements are accurate to avoid discrepancies in your final solution concentration.
• For best results, use calibrated pipettes and fresh reagents.